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KMID : 0861020200350010009
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Korea Journal of Herbology
2020 Volume.35 No. 1 p.9 ~ p.18
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Comparative study on the external micro-morphology of 3 kinds of minute pollen and spore Herbs (Pini Pollen, Typhae Pollen, Lygodii Spora) utilizing scanning electron microscope
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Kim Young-Sik
Song Jun-Ho
Choi Go-Ya
Lee Guem-San
Ju Young-Sung
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Abstract
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Objectives：We tried to establish standards for genuine by discriminating 3 kinds of pollen and spore herbs that are highly to be mixed with each other. Using an scanning electron microscope, we prepare a standard for discriminating external morphological characters of minute herbs, thereby contributing to the stable supply of genuine herbal medicines.
Methods：Standard samples were confirmed by literature review on external morphological characteristics of original plants and herbal medicines, and collection and identification of original plants. The herbal medicines on the market were purchased and classified with using naked eye observation and magnifier. Finally, micromorphological identifications were conducted using an scanning electron microscope.
Results：1. Pini Pollen was clearly distinguished by its relatively medium size and a pollen grain with two swollen reticulate sacci at both ends. The verrucate ornamentation on the exine surface of the corpus and a sunken leptoma germ pore may be used as a discrimination criteria.
2. Typhae Pollen was distinguished by its relatively small size and a saccus on the end of a pollen grain. Reticulate ornamentation of exine surface of the corpus, and a slightly clear ulcerate germ pore can be used as a discrimination criteria.
3. Lygodii Spora was distinguished by its relatively large triangular-ovate shape and trichotomous fissure. Verrucate- tuberculate ornamentation of exine surface and trilete aperture could be used as a discrimination criteria.
Conclusion：These results indicate that the use of electron microscopy is very effective for discriminating the external morphology of minute herbal medicines.
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KEYWORD
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Comparative study, external morphology, minute Herb, electron microscope
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